› 1wdi

X-ray diffraction
2.1Å resolution

Crystal Structure Of TT0907 From Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb1wdi/pdb
PDB entry 1wdi coloured by chain, front view.
PDB entry 1wdi coloured by chain, side view.
PDB entry 1wdi coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Idaka M, Yagi H, Murayama K, Terada T, Kuramitsu S, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine:tRNA ribosyltransferase-isomerase Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 38.48 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72JS5 (Residues: 1-345; Coverage: 100%)
Gene names: TT_C0693, queA
Sequence domains: Queuosine biosynthesis protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21212
Unit cell:
a: 76.791Å b: 77.275Å c: 73.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.27
Expression system: Escherichia coli

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