› 1wek

X-ray diffraction
2.2Å resolution

Crystal structure of the conserved hypothetical protein TT1465 from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1wek/pdb
PDB entry 1wek coloured by chain, front view.
PDB entry 1wek coloured by chain, side view.
PDB entry 1wek coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of possible lysine decarboxylases from Thermus thermophilus HB8.
Kukimoto-Niino M, Murayama K, Kato-Murayama M, Idaka M, Bessho Y, Tatsuguchi A, Ushikoshi-Nakayama R, Terada T, Kuramitsu S, Shirouzu M, Yokoyama S
Protein Sci 13 3038-42 (2004)
PMID: 15459330

Function and Biology Details

Reaction catalysed: 
N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H(2)O = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytokinin riboside 5'-monophosphate phosphoribohydrolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 217 amino acids
Theoretical weight: 24.4 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SHT6 (Residues: 1-217; Coverage: 100%)
Gene name: TTHA1644
Sequence domains: Possible lysine decarboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO4 12 x PO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 65.65Å b: 83.798Å c: 265.143Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Function and structure of the molybdenum cofactor carrier protein from Chlamydomonas reinhardtii.
Fischer et al. (2006)
6 more

4 mentions without citation

Comparative genomic analyses reveal a vast, novel network of nucleotide-centric systems in biological conflicts, immunity and signaling.
Burroughs et al. (2015)
3 more