› 1wg8

X-ray diffraction
2Å resolution

Crystal structure of a predicted S-adenosylmethionine-dependent methyltransferase TT1512 from Thermus thermophilus HB8.

Released:
DOI: 10.2210/pdb1wg8/pdb
PDB entry 1wg8 coloured by chain, front view.
PDB entry 1wg8 coloured by chain, side view.
PDB entry 1wg8 coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Kishishita S, Murayama K, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase H Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 31.22 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SJD8 (Residues: 1-285; Coverage: 100%)
Gene names: TTHA1076, mraW, rsmH
Sequence domains: MraW methylase family
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 68.054Å b: 88.909Å c: 104.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.263
Expression system: Escherichia coli BL21(DE3)

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