› 1wkc

X-ray diffraction
1.7Å resolution

Crystal structure of a 5-formyltetrahydrofolate cycloligase-related protein from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1wkc/pdb
PDB entry 1wkc coloured by chain, front view.
PDB entry 1wkc coloured by chain, side view.
PDB entry 1wkc coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Yanai H, Tsuge H, Utsunomiya H, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-formyltetrahydrofolate cyclo-ligase Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.46 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SHW9 (Residues: 1-184; Coverage: 100%)
Gene name: TTHA1611
Sequence domains: 5-formyltetrahydrofolate cyclo-ligase family
Structure domains: NagB/RpiA/CoA transferase-like

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: I222
Unit cell:
a: 54.01Å b: 73.37Å c: 107.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.251
Expression system: Escherichia coli

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