› 1wl8

X-ray diffraction
1.45Å resolution

Crystal structure of PH1346 protein from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb1wl8/pdb
PDB entry 1wl8 coloured by chain, front view.
PDB entry 1wl8 coloured by chain, side view.
PDB entry 1wl8 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Lokanath NK, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GMP synthase [glutamine-hydrolyzing] subunit A Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.54 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O59071 (Residues: 1-189; Coverage: 100%)
Gene names: PH1346, guaAA
Sequence domains: Glutamine amidotransferase class-I
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand ACY 1 x ACY
2 modified residues:
Ligand SNN 1 x SNN
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 46.808Å b: 63.48Å c: 65.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.174
Expression system: Escherichia coli BL21(DE3)

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