› 1wmd

X-ray diffraction
1.3Å resolution

Crystal Structure of alkaline serine protease KP-43 from Bacillus sp. KSM-KP43 (1.30 angstrom, 100 K)

Released:
DOI: 10.2210/pdb1wmd/pdb
Model geometry
Fit model/data
PDB entry 1wmd coloured by chain, front view.
PDB entry 1wmd coloured by chain, side view.
PDB entry 1wmd coloured by chain, top view.
Source organism: Bacillus sp. KSM-KP43
Primary publication:
The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43, with a C-terminal beta-barrel domain.
Nonaka T, Fujihashi M, Kita A, Saeki K, Ito S, Horikoshi K, Miki K
J Biol Chem 279 47344-51 (2004)
PMID: 15342641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 45.34 KDa
Source organism: Bacillus sp. KSM-KP43
UniProt:
  • Canonical: Q93UV9 (Residues: 207-640; Coverage: 71%)
Gene name: PROF
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 3 x CA
Ligand SO4 1 x SO4
Ligand DIO 2 x DIO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: C2221
Unit cell:
a: 42.84Å b: 109.036Å c: 166.912Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 not available 0.169

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Citations

3 review citations

An overview of Bacillus proteases: from production to application.
Contesini et al. (2018)
2 more

7 mentions without citation

Protein flexibility in the light of structural alphabets.
Craveur et al. (2015)
6 more