› 1wn7

X-ray diffraction
2.75Å resolution

Crystal structure of archaeal family B DNA polymerase mutant

Released:
DOI: 10.2210/pdb1wn7/pdb
PDB entry 1wn7 coloured by chain, front view.
PDB entry 1wn7 coloured by chain, side view.
PDB entry 1wn7 coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Structural mechanism for coordination of proofreading and polymerase activities in archaeal DNA polymerases.
Kuroita T, Matsumura H, Yokota N, Kitabayashi M, Hashimoto H, Inoue T, Imanaka T, Kai Y
J Mol Biol 351 291-8 (2005)
PMID: 16019029

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 774 amino acids
Theoretical weight: 90.15 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWU9 (Residues: 1-774; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 74.344Å b: 111.131Å c: 111.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.226 0.306
Expression system: Escherichia coli

Quick links

Citations

5 review citations

DNA polymerases engineered by directed evolution to incorporate non-standard nucleotides.
Laos et al. (2014)
4 more

2 mentions without citation

Evolution of DNA polymerases: an inactivated polymerase-exonuclease module in Pol epsilon and a chimeric origin of eukaryotic polymerases from two classes of archaeal ancestors.
Tahirov et al. (2009)
1 more