› 1wnf

X-ray diffraction
2.5Å resolution

Crystal Structure of PH0066 from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb1wnf/pdb
PDB entry 1wnf coloured by chain, front view.
PDB entry 1wnf coloured by chain, side view.
PDB entry 1wnf coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Ihsanawati, Sekine S, Murayama K, Sugawara M, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
asparaginase Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 36.28 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O57797 (Residues: 1-328; Coverage: 100%)
Gene name: PH0066
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21212
Unit cell:
a: 125.23Å b: 77.14Å c: 98.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.251
Expression system: Escherichia coli

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