› 1wni

X-ray diffraction
2.2Å resolution

Crystal Structure of H2-Proteinase

Released:
DOI: 10.2210/pdb1wni/pdb
PDB entry 1wni coloured by chain, front view.
PDB entry 1wni coloured by chain, side view.
PDB entry 1wni coloured by chain, top view.
Source organism: Protobothrops flavoviridis
Primary publication:
Crystal structure of H2-proteinase from the venom of Trimeresurus flavoviridis.
Kumasaka T, Yamamoto M, Moriyama H, Tanaka N, Sato M, Katsube Y, Yamakawa Y, Omori-Satoh T, Iwanaga S, Ueki T
J Biochem 119 49-57 (1996)
PMID: 8907175

Function and Biology Details

Reaction catalysed: 
Cleavage of 3-Asn-|-Gln-4, 10-His-|-Leu-11 and 14-Ala-|-Leu-15 in the insulin B chain, and the bond Z-Gly-Pro-|-Leu-Gly-Pro in a small molecule substrate of microbial collagenase.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Snake venom metalloproteinase trimerelysin-2 Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 23.05 KDa
Source organism: Protobothrops flavoviridis
UniProt:
  • Canonical: P20165 (Residues: 1-201; Coverage: 100%)
Sequence domains: Reprolysin (M12B) family zinc metalloprotease
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P43212
Unit cell:
a: 77.8Å b: 77.8Å c: 82.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 not available not available

Quick links

Citations

11 review citations

Structural considerations of the snake venom metalloproteinases, key members of the M12 reprolysin family of metalloproteinases.
Fox et al. (2005)
10 more

4 mentions without citation

ADAM and ADAMTS Family Proteins and Snake Venom Metalloproteinases: A Structural Overview.
Takeda S. (2016)
3 more