› 1wo8

X-ray diffraction
1.7Å resolution

Crystal structure of methylglyoxal synthase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1wo8/pdb
PDB entry 1wo8 coloured by chain, front view.
PDB entry 1wo8 coloured by chain, side view.
PDB entry 1wo8 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Sugahara M, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Glycerone phosphate = methylglyoxal + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer (preferred)
homo dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-177864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylglyoxal synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 126 amino acids
Theoretical weight: 13.4 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SHD6 (Residues: 1-126; Coverage: 100%)
Gene names: TTHA1794, mgsA
Sequence domains: MGS-like domain
Structure domains: Methylglyoxal synthase-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P3121
Unit cell:
a: 130.612Å b: 130.612Å c: 96.653Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.185 0.204
Expression system: Escherichia coli

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