› 1woy

X-ray diffraction
2Å resolution

Crystal structure of methionyl tRNA synthetase Y225F mutant from Thermus thermophilus

Released:
DOI: 10.2210/pdb1woy/pdb
PDB entry 1woy coloured by chain, front view.
PDB entry 1woy coloured by chain, side view.
PDB entry 1woy coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Konno M, Takeda R, Nureki O, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 58 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23395 (Residues: 1-500; Coverage: 81%)
Gene names: TTHA1298, metG, metS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 64.416Å b: 82.731Å c: 141.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.226
Expression system: Escherichia coli BL21(DE3)

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