› 1wpu

X-ray diffraction
1.48Å resolution

Crystal Structure of the HutP antitermination complex bound to a single stranded region of hut mRNA

Released:
DOI: 10.2210/pdb1wpu/pdb
PDB entry 1wpu coloured by chain, front view.
PDB entry 1wpu coloured by chain, side view.
PDB entry 1wpu coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kumarevel TS, Mizuno H, Kumar PKR

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145535 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Hut operon positive regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.1 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P10943 (Residues: 2-148; Coverage: 99%)
Gene names: BSU39340, hutP
Sequence domains: HutP
Structure domains: Hut operon regulatory protein HutP
5'-R(*UP*UP*GP*AP*GP*UP*U)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 nucleotides
Theoretical weight: 2.2 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand HIS 2 x HIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: R3
Unit cell:
a: 76.06Å b: 76.06Å c: 133.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.238
Expression systems:
  • Escherichia coli
  • Not provided

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