› 1wpv

X-ray diffraction
1.7Å resolution

Crystal Structure of Activated Binary complex of HutP, an RNA binding anti-termination protein

Released:
DOI: 10.2210/pdb1wpv/pdb
PDB entry 1wpv coloured by chain, front view.
PDB entry 1wpv coloured by chain, side view.
PDB entry 1wpv coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis of HutP-mediated anti-termination and roles of the Mg2+ ion and L-histidine ligand.
Kumarevel T, Mizuno H, Kumar PK
Nature 434 183-91 (2005)
PMID: 15758992

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hut operon positive regulatory protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 147 amino acids
Theoretical weight: 16.1 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P10943 (Residues: 2-148; Coverage: 99%)
Gene names: BSU39340, hutP
Sequence domains: HutP
Structure domains: Hut operon regulatory protein HutP

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand HIS 3 x HIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 77.79Å b: 80.88Å c: 75.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.259
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Termination and antitermination: RNA polymerase runs a stop sign.
Santangelo et al. (2011)
7 more

4 mentions without citation

Metal ion-dependent anti-termination of transcriptional regulation of ribonucleoprotein complexes.
Kumar et al. (2014)
3 more