› 1wq6

X-ray diffraction
2Å resolution

The tetramer structure of the nervy homolgy two (NHR2) domain of AML1-ETO is critical for AML1-ETO'S activity

Released:
DOI: 10.2210/pdb1wq6/pdb
PDB entry 1wq6 coloured by chain, front view.
PDB entry 1wq6 coloured by chain, side view.
PDB entry 1wq6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The tetramer structure of the Nervy homology two domain, NHR2, is critical for AML1/ETO's activity.
Liu Y, Cheney MD, Gaudet JJ, Chruszcz M, Lukasik SM, Sugiyama D, Lary J, Cole J, Dauter Z, Minor W, Speck NA, Bushweller JH
Cancer Cell 9 249-60 (2006)
PMID: 16616331

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein CBFA2T1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 9.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06455 (Residues: 335-403; Coverage: 11%)
Gene names: AML1T1, CBFA2T1, CDR, ETO, MTG8, RUNX1T1, ZMYND2
Sequence domains: NHR2 domain like
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21212
Unit cell:
a: 60.72Å b: 75.442Å c: 30.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.213 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Histone deacetylases and their inhibitors in cancer, neurological diseases and immune disorders.
Falkenberg et al. (2014)
26 more

3 mentions without citation

New insights into transcriptional and leukemogenic mechanisms of AML1-ETO and E2A fusion proteins.
Li et al. (2016)
2 more