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X-ray diffraction
1.45Å resolution

Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Ot3 in complex with Biotinyl-5-AMP

Released:

Function and Biology Details

Reaction catalysed:
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-129627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BPL/LPL catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.1 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57883 (Residues: 1-235; Coverage: 100%)
Gene name: PH0147
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 38.348Å b: 82.832Å c: 72.785Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.212 0.212 0.24
Expression system: Escherichia coli