› 1wr8

X-ray diffraction
1.6Å resolution

Crystal structure of hypothetical protein PH1421 from Pyrococcus horikoshii.

Released:
DOI: 10.2210/pdb1wr8/pdb
PDB entry 1wr8 coloured by chain, front view.
PDB entry 1wr8 coloured by chain, side view.
PDB entry 1wr8 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structure of a haloacid dehalogenase superfamily phosphatase PH1421 from Pyrococcus horikoshii OT3: oligomeric state and thermoadaptation mechanism.
Yamamoto H, Takio K, Sugahara M, Kunishima N
Acta Crystallogr D Biol Crystallogr 64 1068-77 (2008)
PMID: 18931414

Function and Biology Details

Reaction catalysed: 
2-phosphoglycolate + H(2)O = glycolate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycolate phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 25.64 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O50129 (Residues: 1-231; Coverage: 100%)
Gene name: PH1421
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 63.795Å b: 81.772Å c: 86.949Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.212
Expression system: Escherichia coli

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