› 1wrb

X-ray diffraction
2.4Å resolution

Crystal structure of the N-terminal RecA-like domain of DjVLGB, a pranarian Vasa-like RNA helicase

Released:
DOI: 10.2210/pdb1wrb/pdb
PDB entry 1wrb coloured by chain, front view.
PDB entry 1wrb coloured by chain, side view.
PDB entry 1wrb coloured by chain, top view.
Source organism: Dugesia japonica
Primary publication:
Crystal structure of the N-terminal RecA-like domain of a DEAD-box RNA helicase, the Dugesia japonica vasa-like gene B protein.
Kurimoto K, Muto Y, Obayashi N, Terada T, Shirouzu M, Yabuki T, Aoki M, Seki E, Matsuda T, Kigawa T, Okumura H, Tanaka A, Shibata N, Kashikawa M, Agata K, Yokoyama S
J Struct Biol 150 58-68 (2005)
PMID: 15797730

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.49 KDa
Source organism: Dugesia japonica
Expression system: Not provided
UniProt:
  • Canonical: O97032 (Residues: 162-414; Coverage: 32%)
Gene name: DjvlgB
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P41212
Unit cell:
a: 138.242Å b: 138.242Å c: 47.508Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.282
Expression system: Not provided

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Citations

1 review citation

Two different evolutionary origins of stem cell systems and their molecular basis.
Agata et al. (2006)
8 other articles