› 1ws8

X-ray diffraction
1.6Å resolution

Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)

Released:
DOI: 10.2210/pdb1ws8/pdb
PDB entry 1ws8 coloured by chain, front view.
PDB entry 1ws8 coloured by chain, side view.
PDB entry 1ws8 coloured by chain, top view.
Source organism: Cucurbita pepo
Primary publication:
Structural reorganization of the copper binding site involving Thr15 of mavicyanin from Cucurbita pepo medullosa (zucchini) upon reduction.
Xie Y, Inoue T, Miyamoto Y, Matsumura H, Kataoka K, Yamaguchi K, Nojini M, Suzuki S, Kai Y
J Biochem 137 455-61 (2005)
PMID: 15858169

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mavicyanin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 109 amino acids
Theoretical weight: 11.89 KDa
Source organism: Cucurbita pepo
Expression system: Escherichia coli
UniProt:
  • Canonical: P80728 (Residues: 1-108; Coverage: 100%)
Sequence domains: Plastocyanin-like domain
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 4 x CU
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B, SPRING-8 BEAMLINE BL40B2
Spacegroup: P61
Unit cell:
a: 63.92Å b: 63.92Å c: 245.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.193 0.211
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The role of ligand-containing loops at copper sites in proteins.
Dennison C. (2008)
3 other articles