› 1wsj

X-ray diffraction
2Å resolution

Crystal structure of E.coli RNase HI active site mutant (K87A/H124A)

Released:
DOI: 10.2210/pdb1wsj/pdb
PDB entry 1wsj coloured by chain, front view.
PDB entry 1wsj coloured by chain, side view.
PDB entry 1wsj coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Tsunaka Y, Takano K, Matsumura H, Yamagata Y, Kanaya S

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 155 amino acids
Theoretical weight: 17.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Y4 (Residues: 1-155; Coverage: 100%)
Gene names: JW0204, b0214, dasF, herA, rnh, rnhA, sdrA
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 55.76Å b: 75.078Å c: 147.867Å
α: 90° β: 90.98° γ: 90°
R-values:
R R work R free
0.235 0.235 0.288
Expression system: Escherichia coli

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