1wty

X-ray diffraction
2.2Å resolution

Crystal structure of a probable nucleotidyl transferase protein from thermus thermophilus HB8

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Kitamura Y, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleotidyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 119 amino acids
Theoretical weight: 14.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SM95 (Residues: 1-119; Coverage: 100%)
Gene name: TTHA0048
Sequence domains: Nucleotidyltransferase substrate binding protein like
Structure domains: Nucleotidyltransferases domain 2

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 44.32Å b: 116.25Å c: 123.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.23 0.28
Expression system: Escherichia coli