› 1wud

X-ray diffraction
2.2Å resolution

E. coli RecQ HRDC domain

Released:
DOI: 10.2210/pdb1wud/pdb
PDB entry 1wud coloured by chain, front view.
PDB entry 1wud coloured by chain, side view.
PDB entry 1wud coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conferring substrate specificity to DNA helicases: role of the RecQ HRDC domain.
Bernstein DA, Keck JL
Structure 13 1173-82 (2005)
PMID: 16084389

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase RecQ Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 89 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15043 (Residues: 524-609; Coverage: 14%)
Gene names: JW5855, b3822, recQ
Sequence domains: HRDC domain
Structure domains: HRDC domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P212121
Unit cell:
a: 38.304Å b: 64.611Å c: 98.114Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

RecQ helicases: multifunctional genome caretakers.
Chu et al. (2009)
17 more

5 mentions without citation

ApoE mimetic peptide decreases Abeta production in vitro and in vivo.
Minami et al. (2010)
4 more