› 1wue

X-ray diffraction
2.1Å resolution

Crystal structure of protein GI:29375081, unknown member of enolase superfamily from enterococcus faecalis V583

Released:
DOI: 10.2210/pdb1wue/pdb
PDB entry 1wue coloured by chain, front view.
PDB entry 1wue coloured by chain, side view.
PDB entry 1wue coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Primary publication:
Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero D, Sakai A, Patskovsky Y, Malashkevich VN, Fedorov AA, Bonanno JB, Fedorov EV, Toro R, Agarwal R, Wang C, Ozerova ND, Yew WS, Sauder JM, Swaminathan S, Burley SK, Almo SC, Glasner ME
Proc Natl Acad Sci U S A 111 8535-40 (2014)
PMID: 24872444

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 43.96 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli
UniProt:
  • Canonical: Q838J7 (Residues: 1-367; Coverage: 100%)
Gene names: EF_0450, menC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: R3
Unit cell:
a: 136.807Å b: 136.807Å c: 112.771Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.207 0.242
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
2 more

3 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
2 more