› 1wuf

X-ray diffraction
2.9Å resolution

Crystal structure of protein GI:16801725, member of Enolase superfamily from Listeria innocua Clip11262

Released:
DOI: 10.2210/pdb1wuf/pdb
PDB entry 1wuf coloured by chain, front view.
PDB entry 1wuf coloured by chain, side view.
PDB entry 1wuf coloured by chain, top view.
Source organism: Listeria innocua Clip11262
Primary publication:
Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero D, Sakai A, Patskovsky Y, Malashkevich VN, Fedorov AA, Bonanno JB, Fedorov EV, Toro R, Agarwal R, Wang C, Ozerova ND, Yew WS, Sauder JM, Swaminathan S, Burley SK, Almo SC, Glasner ME
Proc Natl Acad Sci U S A 111 8535-40 (2014)
PMID: 24872444

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 44.47 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli
UniProt:
  • Canonical: Q927X3 (Residues: 1-374; Coverage: 100%)
Gene names: lin2664, menC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 54.721Å b: 87.346Å c: 161.489Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.236 0.274
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
2 more

2 mentions without citation

The LabelHash algorithm for substructure matching.
Moll et al. (2010)
1 more