› 1wuo

X-ray diffraction
2.01Å resolution

Crystal structure of metallo-beta-lactamase IMP-1 mutant (D81A)

Released:
DOI: 10.2210/pdb1wuo/pdb
PDB entry 1wuo coloured by chain, front view.
PDB entry 1wuo coloured by chain, side view.
PDB entry 1wuo coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography.
Yamaguchi Y, Kuroki T, Yasuzawa H, Higashi T, Jin W, Kawanami A, Yamagata Y, Arakawa Y, Goto M, Kurosaki H
J Biol Chem 280 20824-32 (2005)
PMID: 15788415

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 228 amino acids
Theoretical weight: 25.13 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52699 (Residues: 19-246; Coverage: 100%)
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand ACY 4 x ACY
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P1
Unit cell:
a: 49.734Å b: 73.016Å c: 82.45Å
α: 85.37° β: 75.53° γ: 73.62°
R-values:
R R work R free
0.212 0.212 0.218
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Metallo-β-lactamase structure and function.
Palzkill T. (2013)
8 more

1 mention without citation

Evolving carbapenemases: can medicinal chemists advance one step ahead of the coming storm?
Oelschlaeger et al. (2010)