› 1wv2

X-ray diffraction
2.9Å resolution

Crystal structure of thiamine biosynthesis protein from Pseudomonas Aeruginosa

Released:
DOI: 10.2210/pdb1wv2/pdb
PDB entry 1wv2 coloured by chain, front view.
PDB entry 1wv2 coloured by chain, side view.
PDB entry 1wv2 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Fedorov AA, Fedorov EV, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-191452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiazole synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 28.26 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I6B4 (Residues: 1-265; Coverage: 100%)
Gene names: PA0381, thiG
Sequence domains: Thiazole biosynthesis protein ThiG
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3121
Unit cell:
a: 74.115Å b: 74.115Å c: 164.947Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.254 0.304
Expression system: Escherichia coli

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