1wvl

X-ray diffraction
2.6Å resolution

Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-114781 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-binding protein 7d Chains: A, B
Molecule details ›
Chains: A, B
Length: 80 amino acids
Theoretical weight: 9.22 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13123 (Residues: 1-66; Coverage: 100%)
Gene name: Saci_0064
Sequence domains: 7kD DNA-binding domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
5'-D(*CP*CP*TP*AP*TP*AP*TP*AP*GP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P4122
Unit cell:
a: 58.65Å b: 58.65Å c: 186.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.23 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided