› 1wvm

X-ray diffraction
1.6Å resolution

Crystal Structure of Psychrophilic Subtilisin-like Serine Protease APA1 from Antarctic Psychrotroph Pseudoaleromonas sp. AS-11, Complexed with Inhibitor Chymostatin

Released:
DOI: 10.2210/pdb1wvm/pdb
PDB entry 1wvm coloured by chain, front view.
PDB entry 1wvm coloured by chain, side view.
PDB entry 1wvm coloured by chain, top view.
Source organisms:
Entry authors: Dong D, Watanabe K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
homo dimer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162533 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
P/Homo B domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 45.15 KDa
Source organism: Pseudoalteromonas sp. AS-11
UniProt:
  • Canonical: Q65Z69 (Residues: 145-585; Coverage: 56%)
Gene name: apa1
Sequence domains:
Structure domains:
CHYMOSTATIN Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 608 Da
Source organism: Streptomyces hygroscopicus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand MG 8 x MG
1 modified residue:
Ligand PHA 2 x PHA

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 123.23Å b: 138.34Å c: 64.88Å
α: 90° β: 97.56° γ: 90°
R-values:
R R work R free
0.192 0.192 0.206
Expression system: Not provided

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