› 1wvv

X-ray diffraction
2Å resolution

Crystal structure of chitinase C mutant E147Q

Released:
DOI: 10.2210/pdb1wvv/pdb
PDB entry 1wvv coloured by chain, front view.
PDB entry 1wvv coloured by chain, side view.
PDB entry 1wvv coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037.
Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T
J Mol Biol 358 472-84 (2006)
PMID: 16516924

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-129307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitin-binding type-3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 28.5 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50152 (Residues: 30-294; Coverage: 100%)
Gene names: NCTC13033_02762, chiC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 13 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P213
Unit cell:
a: 127.902Å b: 127.902Å c: 127.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The chitinolytic machinery of Serratia marcescens--a model system for enzymatic degradation of recalcitrant polysaccharides.
Vaaje-Kolstad et al. (2013)
3 more

2 mentions without citation

Detection, characterization and evolution of internal repeats in Chitinases of known 3-D structure.
Sivaji et al. (2014)
1 more