› 1ww8

X-ray diffraction
2.5Å resolution

Crystal Structure of malic enzyme from Pyrococcus horikoshii Ot3

Released:
DOI: 10.2210/pdb1ww8/pdb
PDB entry 1ww8 coloured by chain, front view.
PDB entry 1ww8 coloured by chain, side view.
PDB entry 1ww8 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Lokanath NK, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
439aa long hypothetical malate oxidoreductase (NAD) [malic enzyme] Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 48.34 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59029 (Residues: 1-439; Coverage: 100%)
Gene name: PH1275
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 57.457Å b: 85.681Å c: 172.786Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.24
Expression system: Escherichia coli

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