› 1wwk

X-ray diffraction
1.9Å resolution

Crystal structure of phosphoglycerate dehydrogenase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1wwk/pdb
PDB entry 1wwk coloured by chain, front view.
PDB entry 1wwk coloured by chain, side view.
PDB entry 1wwk coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Sugahara M, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
307aa long hypothetical phosphoglycerate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.7 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O50095 (Residues: 1-307; Coverage: 100%)
Gene name: PH1387
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1, RIGAKU
Spacegroup: C2
Unit cell:
a: 222.417Å b: 46.163Å c: 66.258Å
α: 90° β: 92.53° γ: 90°
R-values:
R R work R free
0.224 0.224 0.246
Expression system: Escherichia coli

Quick links