› 1wxw

X-ray diffraction
2.55Å resolution

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

Released:
DOI: 10.2210/pdb1wxw/pdb
PDB entry 1wxw coloured by chain, front view.
PDB entry 1wxw coloured by chain, side view.
PDB entry 1wxw coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-L-homocysteine.
Pioszak AA, Murayama K, Nakagawa N, Ebihara A, Kuramitsu S, Shirouzu M, Yokoyama S
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 867-74 (2005)
PMID: 16511182

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytosine(1962) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1962) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase I Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 382 amino acids
Theoretical weight: 43.25 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIT4 (Residues: 1-382; Coverage: 100%)
Gene name: TTHA1280
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HEZ 1 x HEZ
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 252.704Å b: 45.962Å c: 139.64Å
α: 90° β: 100.08° γ: 90°
R-values:
R R work R free
0.269 0.223 0.279
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein universe containing a PUA RNA-binding domain.
Cerrudo et al. (2014)
3 other articles

2 mentions without citation

Identification and characterization of the Thermus thermophilus 5-methylcytidine (m5C) methyltransferase modifying 23 S ribosomal RNA (rRNA) base C1942.
Larsen et al. (2012)
1 more