› 1wxx

X-ray diffraction
1.8Å resolution

Crystal structure of Tt1595, a putative SAM-dependent methyltransferase from Thermus thermophillus HB8

Released:
DOI: 10.2210/pdb1wxx/pdb
PDB entry 1wxx coloured by chain, front view.
PDB entry 1wxx coloured by chain, side view.
PDB entry 1wxx coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-L-homocysteine.
Pioszak AA, Murayama K, Nakagawa N, Ebihara A, Kuramitsu S, Shirouzu M, Yokoyama S
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 867-74 (2005)
PMID: 16511182

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytosine(1962) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1962) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase I Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 382 amino acids
Theoretical weight: 43.25 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIT4 (Residues: 1-382; Coverage: 100%)
Gene name: TTHA1280
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand K 4 x K
Ligand PO4 8 x PO4
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P1
Unit cell:
a: 77.551Å b: 82.318Å c: 79.616Å
α: 96.34° β: 90.14° γ: 103.06°
R-values:
R R work R free
0.216 0.203 0.229
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein universe containing a PUA RNA-binding domain.
Cerrudo et al. (2014)
3 other articles

1 mention without citation

Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-L-homocysteine.
Pioszak et al. (2005)