› 1wy9

X-ray diffraction
2.1Å resolution

Crystal structure of microglia-specific protein, Iba1

Released:
DOI: 10.2210/pdb1wy9/pdb
PDB entry 1wy9 coloured by chain, front view.
PDB entry 1wy9 coloured by chain, side view.
PDB entry 1wy9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
X-ray structures of the microglia/macrophage-specific protein Iba1 from human and mouse demonstrate novel molecular conformation change induced by calcium binding.
Yamada M, Ohsawa K, Imai Y, Kohsaka S, Kamitori S
J Mol Biol 364 449-57 (2006)
PMID: 17011575

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allograft inflammatory factor 1 Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.94 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70200 (Residues: 1-147; Coverage: 100%)
Gene names: Aif1, Iba1
Sequence domains: Allograft inflammatory factor 1
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3221
Unit cell:
a: 44.056Å b: 44.056Å c: 99.144Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.251
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Targeting microglia L-type voltage-dependent calcium channels for the treatment of central nervous system disorders.
Hopp SC. (2021)
1 more

3 mentions without citation

Linking in domain-swapped protein dimers.
Baiesi et al. (2016)
2 more