› 1wz1

X-ray diffraction
1.85Å resolution

Crystal structure of the Fv fragment complexed with dansyl-lysine

Released:
DOI: 10.2210/pdb1wz1/pdb
PDB entry 1wz1 coloured by chain, front view.
PDB entry 1wz1 coloured by chain, side view.
PDB entry 1wz1 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Conformational dynamics of complementarity-determining region H3 of an anti-dansyl Fv fragment in the presence of its hapten.
Nakasako M, Oka T, Mashumo M, Takahashi H, Shimada I, Yamaguchi Y, Kato K, Arata Y
J Mol Biol 351 627-40 (2005)
PMID: 16019026

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233178 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig kappa chain V-II region 26-10 Chain: L
Molecule details ›
Chain: L
Length: 113 amino acids
Theoretical weight: 12.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01631 (Residues: 1-113; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig heavy chain Chain: H
Molecule details ›
Chain: H
Length: 123 amino acids
Theoretical weight: 14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand DNS 1 x DNS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P43212
Unit cell:
a: 81.63Å b: 81.63Å c: 74.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.253
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Biophysical characterization of dynamic structures of immunoglobulin G.
Yanaka et al. (2020)
4 other articles

4 mentions without citation

An anti-hapten camelid antibody reveals a cryptic binding site with significant energetic contributions from a nonhypervariable loop.
Fanning et al. (2011)
3 more