› 1wz2

X-ray diffraction
3.21Å resolution

The crystal structure of Leucyl-tRNA synthetase and tRNA(leucine) complex

Released:
DOI: 10.2210/pdb1wz2/pdb
PDB entry 1wz2 coloured by chain, front view.
PDB entry 1wz2 coloured by chain, side view.
PDB entry 1wz2 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Fukunaga R, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129755 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Leucine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 967 amino acids
Theoretical weight: 114.12 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58698 (Residues: 1-967; Coverage: 100%)
Gene names: PH0965, leuS
Sequence domains:
Structure domains:
tRNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 88 nucleotides
Theoretical weight: 28.38 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21212
Unit cell:
a: 120.546Å b: 231.127Å c: 118.181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.305
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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