› 1wz8

X-ray diffraction
1.8Å resolution

Crystal Structure of Probable Enoyl-CoA Dehydratase from Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb1wz8/pdb
PDB entry 1wz8 coloured by chain, front view.
PDB entry 1wz8 coloured by chain, side view.
PDB entry 1wz8 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Bagautdinov B, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178213 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 264 amino acids
Theoretical weight: 28.84 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLS5 (Residues: 1-264; Coverage: 100%)
Gene name: TTHA0218
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MPD 8 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 76.485Å b: 127.065Å c: 146.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.258
Expression system: Escherichia coli BL21(DE3)

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