› 1wzo

X-ray diffraction
1.9Å resolution

Crystal Structure of the HpcE from Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb1wzo/pdb
PDB entry 1wzo coloured by chain, front view.
PDB entry 1wzo coloured by chain, side view.
PDB entry 1wzo coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarylacetoacetase-like C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 27.76 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SJQ0 (Residues: 1-246; Coverage: 100%)
Gene name: TTHA0958
Sequence domains: Fumarylacetoacetate (FAA) hydrolase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand GOL 6 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21212
Unit cell:
a: 126.353Å b: 149.415Å c: 65.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links