› 1x0g

X-ray diffraction
2.5Å resolution

Crystal Structure of IscA with the [2Fe-2S] cluster

Released:
DOI: 10.2210/pdb1x0g/pdb
Model geometry
Fit model/data
PDB entry 1x0g coloured by chain, front view.
PDB entry 1x0g coloured by chain, side view.
PDB entry 1x0g coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold.
Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M
J Mol Biol 360 117-32 (2006)
PMID: 16730357

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FeS cluster biogenesis domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 112 amino acids
Theoretical weight: 12.37 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DLM0 (Residues: 1-112; Coverage: 100%)
Gene name: tll0464
Sequence domains: Iron-sulphur cluster biosynthesis
Structure domains: HesB-like domain

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I222
Unit cell:
a: 77.472Å b: 102.788Å c: 137.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.242
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Fe-S cluster assembly pathways in bacteria.
Ayala-Castro et al. (2008)
6 more

7 mentions without citation

Fe-S cluster biogenesis by the bacterial Suf pathway.
Blahut et al. (2020)
6 more