› 1x1q

X-ray diffraction
2.5Å resolution

Crystal structure of tryptophan synthase beta chain from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1x1q/pdb
PDB entry 1x1q coloured by chain, front view.
PDB entry 1x1q coloured by chain, side view.
PDB entry 1x1q coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase beta chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 45.87 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16609 (Residues: 1-404; Coverage: 100%)
Gene names: TT_C0730, trpB
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21212
Unit cell:
a: 92.187Å b: 109.993Å c: 74.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.245
Expression system: Escherichia coli BL21(DE3)

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