› 1x1t

X-ray diffraction
1.52Å resolution

Crystal Structure of D-3-Hydroxybutyrate Dehydrogenase from Pseudomonas fragi Complexed with NAD+

Released:
DOI: 10.2210/pdb1x1t/pdb
PDB entry 1x1t coloured by chain, front view.
PDB entry 1x1t coloured by chain, side view.
PDB entry 1x1t coloured by chain, top view.
Source organism: Pseudomonas fragi
Primary publication:
D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme.
Ito K, Nakajima Y, Ichihara E, Ogawa K, Katayama N, Nakashima K, Yoshimoto T
J Mol Biol 355 722-33 (2006)
PMID: 16325199

Function and Biology Details

Reaction catalysed: 
(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D(-)-3-hydroxybutyrate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 26.71 KDa
Source organism: Pseudomonas fragi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KST5 (Residues: 1-260; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand MG 1 x MG
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I222
Unit cell:
a: 64.765Å b: 99.97Å c: 109.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.213
Expression system: Escherichia coli

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Citations

1 review citation

[Biochemistry and structural biology of microbial enzymes and their medical applications].
Yoshimoto T. (2007)
15 other articles