› 1x42

X-ray diffraction
2Å resolution

Crystal structure of a haloacid dehalogenase family protein (PH0459) from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1x42/pdb
PDB entry 1x42 coloured by chain, front view.
PDB entry 1x42 coloured by chain, side view.
PDB entry 1x42 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structure of the probable haloacid dehalogenase PH0459 from Pyrococcus horikoshii OT3.
Arai R, Kukimoto-Niino M, Kuroishi C, Bessho Y, Shirouzu M, Yokoyama S
Protein Sci 15 373-7 (2006)
PMID: 16385007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde 3-phosphate phosphatase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 27.09 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58216 (Residues: 1-232; Coverage: 100%)
Gene name: PH0459
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 34.113Å b: 71.272Å c: 53.615Å
α: 90° β: 93.51° γ: 90°
R-values:
R R work R free
0.166 0.166 0.207
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Enzymes that catalyse SN2 reaction mechanisms.
O'Hagan et al. (2010)
4 more

7 mentions without citation

Mini Review: Advances in 2-Haloacid Dehalogenases.
Wang et al. (2021)
6 more