› 1x6n

X-ray diffraction
2Å resolution

Crystal structure of S. marcescens chitinase A mutant W167A in complex with allosamidin

Released:
DOI: 10.2210/pdb1x6n/pdb
PDB entry 1x6n coloured by chain, front view.
PDB entry 1x6n coloured by chain, side view.
PDB entry 1x6n coloured by chain, top view.
Source organism: Serratia marcescens
Entry authors: Aronson Jr NN, Halloran BA, Alexyev MF, Zhou XE, Wang Y, Meehan EJ, Chen L

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase A Chain: A
Molecule details ›
Chain: A
Length: 563 amino acids
Theoretical weight: 60.97 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07254 (Residues: 1-563; Coverage: 100%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAA
Carbohydrate polymer
1 bound ligand:
Ligand AMI 1 x AMI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I222
Unit cell:
a: 76.67Å b: 133.07Å c: 192.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.214 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links