Function and Biology Details
Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
CcdB-poisoned gyrase complex (preferred)
PDBe Complex ID:
PDB-CPX-142493 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA gyrase subunit A
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Structure domains: Topoisomerase, domain 3
Length: 132 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
P0AES4 (Residues: 363-494; Coverage: 15%)
Structure domains: Topoisomerase, domain 3
Toxin CcdB
Molecule details ›
Chains: C, D
Length: 101 amino acids
Theoretical weight: 11.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains: CcdB protein
Structure domains: SH3 type barrels.
Length: 101 amino acids
Theoretical weight: 11.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical: P62554 (Residues: 1-101; Coverage: 100%)
Sequence domains: CcdB protein
Structure domains: SH3 type barrels.
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-1
Spacegroup:
P212121
Expression system: Escherichia coli
