› 1x7i

X-ray diffraction
1.7Å resolution

Crystal structure of the native copper homeostasis protein (cutCm) with calcium binding from Shigella flexneri 2a str. 301

Released:
DOI: 10.2210/pdb1x7i/pdb
PDB entry 1x7i coloured by chain, front view.
PDB entry 1x7i coloured by chain, side view.
PDB entry 1x7i coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 301
Primary publication:
Crystal structure of the copper homeostasis protein (CutCm) from Shigella flexneri at 1.7 A resolution: the first structure of a new sequence family of TIM barrels.
Zhu DY, Zhu YQ, Huang RH, Xiang Y, Yang N, Lu HX, Li GP, Jin Q, Wang DC
Proteins 58 764-8 (2005)
PMID: 15624211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PF03932 family protein CutC Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.86 KDa
Source organism: Shigella flexneri 2a str. 301
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P67825 (Residues: 1-248; Coverage: 100%)
Gene names: S2005, SF1915, cutC, cutCm
Sequence domains: CutC family
Structure domains: Copper homeostasis (CutC) domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2221
Unit cell:
a: 75.327Å b: 97.672Å c: 132.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.226
Expression system: Escherichia coli BL21

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Citations

1 review citation

Research progress in Shigella in the postgenomic era.
Peng et al. (2010)
2 other articles