› 1x7n

X-ray diffraction
1.89Å resolution

The crystal structure of Pyrococcus furiosus phosphoglucose isomerase with bound 5-phospho-D-arabinonate and Manganese

Released:
DOI: 10.2210/pdb1x7n/pdb
PDB entry 1x7n coloured by chain, front view.
PDB entry 1x7n coloured by chain, side view.
PDB entry 1x7n coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
The structures of inhibitor complexes of Pyrococcus furiosus phosphoglucose isomerase provide insights into substrate binding and catalysis.
Berrisford JM, Akerboom J, Brouns S, Sedelnikova SE, Turnbull AP, van der Oost J, Salmon L, Hardré R, Murray IA, Blackburn GM, Rice DW, Baker PJ
J Mol Biol 343 649-57 (2004)
PMID: 15465052

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.64 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83194 (Residues: 1-189; Coverage: 100%)
Gene names: PF0196, pgiA
Sequence domains: Glucose-6-phosphate isomerase (GPI)
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand PA5 1 x PA5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 84.674Å b: 42.382Å c: 57.255Å
α: 90° β: 120.57° γ: 90°
R-values:
R R work R free
0.144 0.141 0.212
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
2 more

2 mentions without citation

Distant Non-Obvious Mutations Influence the Activity of a Hyperthermophilic Pyrococcusfuriosus Phosphoglucose Isomerase.
Subramanian et al. (2019)
1 more