› 1x8m

X-ray diffraction
2.6Å resolution

X-ray structure of pectin degrading enzyme 5-keto 4-deoxyuronate isomerase from Escherichia coli

Released:
DOI: 10.2210/pdb1x8m/pdb
PDB entry 1x8m coloured by chain, front view.
PDB entry 1x8m coloured by chain, side view.
PDB entry 1x8m coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Fedorov AA, Fedorov EV, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
5-dehydro-4-deoxy-D-glucuronate = 3-deoxy-D-glycero-2,5-hexodiulosonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 288 amino acids
Theoretical weight: 32.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46938 (Residues: 2-278; Coverage: 100%)
Gene names: JW2811, b2843, kduI, yqeE
Sequence domains: KduI/IolB family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P31
Unit cell:
a: 101.011Å b: 101.011Å c: 179.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.257 0.28
Expression system: Escherichia coli

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