› 1x8w

X-ray diffraction
3.8Å resolution

Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site

Released:
DOI: 10.2210/pdb1x8w/pdb
PDB entry 1x8w coloured by chain, front view.
PDB entry 1x8w coloured by chain, side view.
PDB entry 1x8w coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site.
Guo F, Gooding AR, Cech TR
Mol Cell 16 351-62 (2004)
PMID: 15525509

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195837 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Tetrahymena ribozyme RNA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 nucleotides
Theoretical weight: 79.97 KDa
Source organism: Tetrahymena thermophila
Expression system: Not provided
Sequence domains: Group I catalytic intron

Ligands and Environments

1 bound ligand:
Ligand MG 38 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P41212
Unit cell:
a: 175.4Å b: 175.4Å c: 304.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.263 0.32
Expression system: Not provided

Quick links

Citations

39 review citations

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
38 more

28 mentions without citation

RNA structure: the long and the short of it.
Holbrook SR. (2005)
27 more