› 1x9q

X-ray diffraction
1.5Å resolution

4m5.3 anti-fluorescein single chain antibody fragment (scFv)

Released:
DOI: 10.2210/pdb1x9q/pdb
PDB entry 1x9q coloured by chain, front view.
PDB entry 1x9q coloured by chain, side view.
PDB entry 1x9q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody.
Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup KD
J Mol Biol 343 685-701 (2004)
PMID: 15465055

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4m5.3 anti-fluorescein single chain antibody fragment Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 29.48 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ACT 5 x ACT
Ligand FLU 1 x FLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 55.78Å b: 57.45Å c: 79.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.165 0.193
Expression system: Saccharomyces cerevisiae

Quick links

Citations

7 review citations

Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
Wells et al. (2007)
6 more

22 mentions without citation

Applications of Yeast Surface Display for Protein Engineering.
Cherf et al. (2015)
21 more