› 1xa9

X-ray diffraction
2.5Å resolution

Crystal structure of yellow fluorescent protein zFP538 K66M green mutant

Released:
DOI: 10.2210/pdb1xa9/pdb
PDB entry 1xa9 coloured by chain, front view.
PDB entry 1xa9 coloured by chain, side view.
PDB entry 1xa9 coloured by chain, top view.
Source organism: Zoanthus sp.
Primary publication:
zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore.
Remington SJ, Wachter RM, Yarbrough DK, Branchaud B, Anderson DC, Kallio K, Lukyanov KA
Biochemistry 44 202-12 (2005)
PMID: 15628861

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein FP538 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.19 KDa
Source organism: Zoanthus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y4 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand BME 1 x BME
1 modified residue:
Ligand CH6 1 x CH6

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6222
Unit cell:
a: 108.432Å b: 108.432Å c: 84.924Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.176 0.295
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
24 more

2 mentions without citation

Gγ and Gα Identity Dictate a G-Protein Heterotrimer Plasma Membrane Targeting.
Mystek et al. (2019)
1 more