› 1xb8

X-ray diffraction
2Å resolution

Zn substituted form of D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin

Released:
DOI: 10.2210/pdb1xb8/pdb
PDB entry 1xb8 coloured by chain, front view.
PDB entry 1xb8 coloured by chain, side view.
PDB entry 1xb8 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Effects of a novel disulfide bond and engineered electrostatic interactions on the thermostability of azurin.
Tigerström A, Schwarz F, Karlsson G, Okvist M, Alvarez-Rúa C, Maeder D, Robb FT, Sjölin L
Biochemistry 43 12563-74 (2004)
PMID: 15449946

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, C
Molecule details ›
Chains: A, C
Length: 128 amino acids
Theoretical weight: 13.92 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: C2
Unit cell:
a: 113.14Å b: 50.038Å c: 57.722Å
α: 90° β: 119.51° γ: 90°
R-values:
R R work R free
0.194 0.192 0.236
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

A study on the effect of surface lysine to arginine mutagenesis on protein stability and structure using green fluorescent protein.
Sokalingam et al. (2012)
8 more